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Article

Improving the affinity of an antibody for its antigen via long-range electrostatic interactions.

Protein engineering, design & selection : PEDS - 1 Dec 2013

Fukunaga Atsushi, Tsumoto Kouhei

Abstract excerpt

To address how long-range electrostatic force can affect antibody-antigen binding, we focused on the interactions between human cardiac troponin I and its specific single-chain antibodies (scFvs). We first isolated two scFvs against two linear epitopes with distinct isoelectric points. For the scFv against the acidic epitope (A1scFv), we mutated five residues of framework region 3 of the light chain to Lys or...

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Source

Identifiers

PMID: 24214686
DOI: 10.1093/protein/gzt053

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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