Article
Deletion of N-glycosylation sites of hepatitis C virus envelope protein E1 enhances specific cellular and humoral immune responses.
Vaccine - 4 Sept 2007
Liu Min, Chen Haidan, Luo Fengling, Li Pingfei, Pan Qin, Xia Bing, Qi Zhongtian, Ho Wen-Zhe, Zhang Xiao-Lian
Abstract excerpt
N-linked glycosylations of viral proteins have been implicated in immunogenicity. In this study, the effects of the N-linked glycosylation of the hepatitis C virus (HCV) E1 protein, a naturally poor immunogen, on the induction of specific immune response were examined. We constructed the plasmids containing the genes encoding both wild type and mutant E1 proteins in which N-linked glycosylation sites are mutated...
Topics
- Amino Acid Sequence
- Animals
- Antibody Formation
- Binding Sites
- Blotting, Western
- CD8-Positive T-Lymphocytes
- Cell Line, Tumor
- Cytokines
- Female
- Glycosylation
- Hepacivirus
- Immunity, Cellular
- Interferon-gamma