Article
Engineering N-glycosylation mutations in IL-12 enhances sustained cytotoxic T lymphocyte responses for DNA immunization.
Nature biotechnology - 1 Apr 2002
Ha Sang J, Chang Jun, Song Man K, Suh You S, Jin Hyun T, Lee Chu H, Nam Gyu H, Choi Gildon, Choi Kwan Y, Lee Sung H, Kim Won B, Sung Young C
Abstract excerpt
Interleukin-12 (IL-12), consisting of p40 and p35 subunits, produces both p70 heterodimer and free p40. p70 is essential for the induction of T-helper 1 (Th1) and cytotoxic T-cell (CTL) immunity, whereas p40 inhibits p70-mediated function. Here, we found that mutations introduced into N-glycosylation sites (N220 of murine p40 and N222 of human p40) reduced secretion of p40 but not p70. Co-immunization of N220...
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