Article
Temporal and functional changes in glycosaminoglycan expression during osteogenesis.
Journal of molecular histology - 1 Oct 2007
Nurcombe Victor, Goh Fuqi Jack, Haupt Larisa M, Murali Sadasivam, Cool Simon M
Abstract excerpt
Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3-E1 mouse calvarial pre-osteoblast cells in order to explore the idea that changes in HS activity...
Topics
- 3T3 Cells
- Animals
- Calcification, Physiologic
- Cell Differentiation
- Cell Proliferation
- Extracellular Matrix
- Fluorescent Antibody Technique
- Glucuronidase
- Glycosaminoglycans
- Heparan Sulfate Proteoglycans
- Heparitin Sulfate
- Immunoblotting
- Mice
- Osteogenesis
- Phenotype
- Reverse Transcriptase Polymerase Chain Reaction
- Sulfotransferases