Article
A SAGA-independent function of SPT3 mediates transcriptional deregulation in a mutant of the Ccr4-not complex in Saccharomyces cerevisiae.
Genetics - 1 Sept 2007
James Nicole, Landrieux Emilie, Collart Martine A
Abstract excerpt
The conserved multi-subunit Ccr4-Not complex regulates gene expression in diverse ways. In this work, we characterize the suppression of temperature sensitivity associated with a mutation in the gene encoding the scaffold subunit of the Ccr4-Not complex, NOT1, by the deletion of SPT3. We determine that the deletion of SPT3, but not the deletion of genes encoding other subunits of the SAGA complex, globally...
Topics
- Blotting, Western
- Cell Cycle Proteins
- Chromatin Immunoprecipitation
- Gene Expression Profiling
- Mutation
- Oligonucleotide Array Sequence Analysis
- RNA, Fungal
- Ribonucleases
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Deletion
- Temperature
- Trans-Activators