Article
Pyrosequencing protocol using a universal biotinylated primer for mutation detection and SNP genotyping.
Nature protocols - 1 Jan 2007
Royo Jose Luis, Hidalgo Manuel, Ruiz Agustin
Abstract excerpt
DNA sequencing has markedly changed the nature of biomedical research, identifying millions of polymorphisms along the human genome that now require further analysis to study the genetic basis of human diseases. Among the DNA-sequencing platforms available, Pyrosequencing has become a useful tool for medium-throughput single nucleotide polymorphism (SNP) genotyping, mutation detection, copy-number studies and DNA...
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