Article
New universal primers facilitate Pyrosequencing.
Electrophoresis - 1 Feb 2006
Aydin Atakan, Toliat Mohammad R, Bähring Sylvia, Becker Christian, Nürnberg Peter
Abstract excerpt
A cost-driving factor in Pyrosequencing is the need for single-stranded PCR products that are usually obtained by biotin-labeling of one primer. We designed new universal primers that allow the introduction of biotin during the specific PCR at either the forward or the reverse primer in a single reaction. When converting five human single nucleotide polymorphism assays from the standard format into the universal...
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