Article
New universal primers facilitate Pyrosequencing.
Electrophoresis - 1 Feb 2006
Aydin Atakan, Toliat Mohammad R, Bähring Sylvia, Becker Christian, Nürnberg Peter
Abstract excerpt
A cost-driving factor in Pyrosequencing is the need for single-stranded PCR products that are usually obtained by biotin-labeling of one primer. We designed new universal primers that allow the introduction of biotin during the specific PCR at either the forward or the reverse primer in a single reaction. When converting five human single nucleotide polymorphism assays from the standard format into the universal...
Topics
- Apolipoproteins
- Biotin
- Caspase 9
- Caspases
- DNA Primers
- Glutamate Decarboxylase
- Humans
- Isoenzymes
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
- Receptors, Corticotropin-Releasing Hormone
- Receptors, GABA-B
- Sequence Analysis, DNA