Method for preparing single-stranded DNA templates for Pyrosequencing using vector ligation and universal biotinylated primers.

Abstract excerpt

In Pyrosequencing, the addition of nucleotides to a primer-template hybrid is monitored by enzymatic conversion of chemical energy into detectable light. The technique yields both qualitative and quantitative sequence information because the chemical energy is released by a stoichiometric split off of pyrophosphates from incorporated deoxynucleotide triphosphates and a defined nucleotide dispensation order is...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Biotinylation
• Share to DNA Primers