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Genomic reduction assisted single nucleotide polymorphism discovery using 454-pyrosequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Maughan Peter J, Udall Joshua A, Jellen Eric N

Abstract excerpt

We report the development of a simple genomic reduction protocol based on 454-pyrosequencing technology that discovers large numbers of single nucleotide polymorphisms (SNP) from pooled DNA samples. The method is based on the conservation of restriction endonuclease sites across samples and biotin separation for genomic reduction and the addition of multiplex identifier (MID) barcodes to each of the pooled...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 25373757
DOI: 10.1007/978-1-4939-1966-6_13

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