Article
Multiplex Pyrosequencing.
Nucleic acids research - 1 Apr 2002
Pourmand Nader, Elahi Elahe, Davis Ronald W, Ronaghi Mostafa
Abstract excerpt
We describe here the development of a new and simple single-tube multiplex Pyrosequencing assay. Genomic DNA or cDNA was employed to PCR amplify region(s) using biotinylated and normal primer(s). Subsequent to capture of PCR products on streptavidin-coated beads, single-stranded DNA separation an...
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