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Article

Universal primer applications for pyrosequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007

Guo Dong-Chuan, Milewicz Dianna M

Abstract excerpt

Pyrosequencing is a high-throughput technique for single-nucleotide polymorphism (SNP) genotyping, DNA sequencing, and SNP allele frequency and DNA methylation assays. The Pyrosequencing assay involves initially purifying PCR-generated single-strand DNA labeled with biotin as a sequencing template. The disadvantage of the synthesis of a biotinylated sequence-specific primer to assay each DNA variant lies in costs...

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PMID: 17185757
DOI: 10.1385/1-59745-377-3:57

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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