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Six new amino acid-auxotrophic markers for targeted gene integration and disruption in fission yeast.

Current genetics - 1 Aug 2007

Ma Yan, Sugiura Reiko, Saito Mariko, Koike Atsushi, Sio Susie Ong, Fujita Yasuko, Takegawa Kaoru, Kuno Takayoshi

Abstract excerpt

Fission yeast Schizosaccharomyces pombe is amenable to genetics and is an excellent model system for studying eukaryotic cell biology. However, auxotrophic markers that can be used for both targeted gene integration and disruption are very limited. Here we performed a forward genetic screen in an effort to develop a new set of selectable markers for use in this yeast. Mutants that were auxotrophic for arginine,...

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PMID: 17622533
DOI: 10.1007/s00294-007-0142-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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