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A set of vectors and strains for chromosomal integration in fission yeast.

Scientific reports - 8 Jun 2023

Matsuyama Akihisa, Hashimoto Atsushi, Nishimura Shinichi, Yoshida Minoru

Abstract excerpt

The expression of heterologous genes is an important technique in yeast genetics. In fission yeast, the leu1 and ura4 genes have been used mainly as selectable markers for heterologous expression. To expand the repertoire of selection markers available for heterologous expression of genes, here we developed new host-vector systems employing lys1 and arg3. By employing genome editing with the CRISPR/Cas9 system,...

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PMID: 37291244
DOI: 10.1038/s41598-023-36267-1
PMCID: PMC10250367

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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