Article
Cloning the Schizosaccharomyces pombe lys2+ gene and construction of new molecular genetic tools.
Current genetics - 1 Jun 2006
Hoffman Richard L, Hoffman Charles S
Abstract excerpt
Molecular genetic analyses in Schizosaccharomyces pombe rely on selectable markers that are used in cloning vectors or to mark targeted gene deletions and other integrated constructs. In this study, we used genetic mapping data and genomic sequence information to predict the identity of the S. pombe lys2(+) gene, which is homologous to Saccharomyces cerevisiae LYS4(+). We confirmed this prediction, showing that...
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