Article
Genome-wide maps of chromatin state in pluripotent and lineage-committed cells.
Nature - 2 Aug 2007
Mikkelsen Tarjei S, Ku Manching, Jaffe David B, Issac Biju, Lieberman Erez, Giannoukos Georgia, Alvarez Pablo, Brockman William, Kim Tae-Kyung, Koche Richard P, Lee William, Mendenhall Eric, O'Donovan Aisling, Presser Aviva, Russ Carsten, Xie Xiaohui, Meissner Alexander, Wernig Marius, Jaenisch Rudolf, Nusbaum Chad, Lander Eric S, Bernstein Bradley E
Abstract excerpt
We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse embryonic stem cells, neural progenitor cells and embryonic fibroblasts. We find that lysine 4 and lysine 27 trimethylation...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.