Article
Studies on the regulation of influenza virus RNA replication: a differential inhibition of the synthesis of vRNA segments in shift-up experiments with ts mutants.
Archives of virology - 1 Jan 1991
Gubareva L V, Varich N L, Markushin S G, Kaverin N V
Abstract excerpt
The regulation of influenza virus vRNA synthesis in the course of the reproduction cycle was studied with the use of a series of ts mutants in shift-up experiments. The synthesis of vRNA segments was registered by means of polyacrylamide gel electrophoresis of nucleocapsid-associated RNA isolated from the infected cells labelled with [3H]uridine after the shift-up to a semi-permissive temperature. Each mutant...
Topics
- Animals
- Capsid
- Chick Embryo
- Influenza A virus
- Kinetics
- Mutation
- Phenotype
- RNA, Viral
- Temperature