Article
Decreasing amplification bias associated with multiple displacement amplification and short tandem repeat genotyping.
Analytical biochemistry - 15 Sept 2007
Ballantyne Kaye N, van Oorschot Roland A H, Muharam Iman, van Daal Angela, John Mitchell R
Abstract excerpt
Although multiple displacement amplification (MDA) is being used increasingly to amplify genomes, the amplification bias generated by the varphi29 polymerase can be a concern with genotyping applications. It has been noted that the bias is pronounced with small template amounts, particularly with single nucleotide polymorphism (SNP) and short tandem repeat (STR) genotyping. Bias may occur between loci, or between...
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