Article
DNA polymerase alpha from HeLa cells synthesizes DNA with high fidelity in a reconstituted replication system.
Mutation research - 1 Oct 1990
Carty M P, Ishimi Y, Levine A S, Dixon K
Abstract excerpt
To determine the contribution that DNA polymerase alpha makes to the overall DNA replication fidelity in mammalian systems, we measured the fidelity of replication of the SV40-based shuttle vector, pZ189, in a reconstituted in vitro DNA replication system which contained purified HeLa DNA polymerase alpha (in addition to single-stranded DNA binding protein, topoisomerase II, DNA ligase, 5'----3' exonuclease,...
Topics
- Cell-Free System
- DNA Polymerase II
- DNA Replication
- Genetic Vectors
- HeLa Cells
- Humans
- In Vitro Techniques
- Mutation