Testing the evolvability of an insect carboxylesterase for the detoxification of synthetic pyrethroid insecticides.

doi:10.1016/j.ibmb.2012.01.004

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Article

Testing the evolvability of an insect carboxylesterase for the detoxification of synthetic pyrethroid insecticides.

Insect biochemistry and molecular biology - 1 May 2012

Coppin Chris W, Jackson Colin J, Sutherland Tara, Hart Peter J, Devonshire Alan L, Russell Robyn J, Oakeshott John G

Abstract excerpt

Esterases have been implicated in metabolic resistance to synthetic pyrethroids in several insect species but little is yet known of the molecular basis for these effects. In this work modern directed evolution technology was used to test to what extent it is possible to genetically enhance the pyrethroid hydrolytic activity of the E3 carboxylesterase from the blowfly Lucilia cuprina. High throughput screening of...

Topics

Amino Acid Substitution
Animals
Carboxylesterase
Diptera
Escherichia coli
Evolution, Molecular
Insect Proteins
Insecticide Resistance
Insecticides
Mutation
Nitriles
Protein Structure, Tertiary
Pyrethrins
Recombination, Genetic

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PMID: 22300675
DOI: 10.1016/j.ibmb.2012.01.004

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