Article
Structural and functional perturbation of Giardia lamblia triosephosphate isomerase by modification of a non-catalytic, non-conserved region.
PloS one - 1 Jan 2013
Hernández-Alcántara Gloria, Torres-Larios Alfredo, Enríquez-Flores Sergio, García-Torres Itzhel, Castillo-Villanueva Adriana, Méndez Sara T, de la Mora-de la Mora Ignacio, Gómez-Manzo Saúl, Torres-Arroyo Angélica, López-Velázquez Gabriel, Reyes-Vivas Horacio, Oria-Hernández Jesús
Abstract excerpt
BACKGROUND: We have previously proposed triosephosphate isomerase of Giardia lamblia (GlTIM) as a target for rational drug design against giardiasis, one of the most common parasitic infections in humans. Since the enzyme exists in the parasite and the host, selective inhibition is a major challenge because essential regions that could be considered molecular targets are highly conserved. Previous biochemical...
Topics
- Biocatalysis
- Conserved Sequence
- Crystallography, X-Ray
- Enzyme Stability
- Giardia lamblia
- Glycolates
- Kinetics
- Models, Molecular
- Mutagenesis, Site-Directed