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Article

Laser photoexcitation of NAD(P)H induces reduction of P450 BM3 heme domain on the microsecond time scale.

Journal of the American Chemical Society - 23 May 2007

Girvan Hazel M, Heyes Derren J, Scrutton Nigel S, Munro Andrew W

Abstract excerpt

We demonstrate that photoexcitation of NAD(P)H at 355 nm using a Nd:YAG laser leads to rapid reduction of the heme domain of the Bacillus megaterium fatty acid hydroxylase flavocytochrome P450 BM3. An aqueous electron derived from photoexcited NAD(P)H is rapidly transferred to the heme domain, enabling the formation of a carbon monoxy complex of the ferrous P450 (FeII-CO) on the microsecond time scale. Using this...

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PMID: 17465554
DOI: 10.1021/ja071355m

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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