Article
Structural and spectroscopic characterization of P450 BM3 mutants with unprecedented P450 heme iron ligand sets. New heme ligation states influence conformational equilibria in P450 BM3.
The Journal of biological chemistry - 5 Jan 2007
Girvan Hazel M, Seward Harriet E, Toogood Helen S, Cheesman Myles R, Leys David, Munro Andrew W
Abstract excerpt
Two novel P450 heme iron ligand sets were generated by directed mutagenesis of the flavocytochrome P450 BM3 heme domain. The A264H and A264K variants produce Cys-Fe-His and Cys-Fe-Lys axial ligand sets, which were validated structurally and characterized by spectroscopic analysis. EPR and magnetic circular dichroism (MCD) provided fingerprints defining these P450 ligand sets. Near IR MCD spectra identified ferric...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.