Article
EMS Mutagenesis of Arabidopsis Seeds.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Gillmor C Stewart, Lukowitz Wolfgang
Abstract excerpt
The ethylating agent ethyl methanesulfonate (EMS) is widely used for inducing random point mutations. In Arabidopsis, treatment with EMS causes GC-to-AT transitions with great efficiency: it has been estimated that a population of 50,000 well-mutagenized plants harbors one or more transitions in almost every GC pair of the genome. These properties, combined with ease of use, make EMS a mutagen of choice for...
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