Article
Suppressor Screens in Arabidopsis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Li Xin, Zhang Yuelin
Abstract excerpt
Genetic screens have proven to be a useful tool in the dissection of biological processes in plants. Specifically, suppressor screens have been widely used to study signal transduction pathways. Here we provide a detailed protocol for ethyl methanesulfonate (EMS) mutagenesis used in our suppressor screens in Arabidopsis and discuss the basic principles behind suppressor screen design and downstream analyses.
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