A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

doi:10.1016/0378-1119(90)90351-q

Back to search

Article

A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

Gene - 30 Nov 1990

Landt O, Grunert H P, Hahn U

Abstract excerpt

We have developed a general and rapid method for site-directed mutagenesis using primed amplification by the polymerase chain reaction. Starting from a double-stranded DNA template, this method requires only one single specific mutagenic primer and two universal sequencing primers flanking the re...

Topics

Base Sequence
Cloning, Molecular
Electrophoresis, Agar Gel
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Polymerase Chain Reaction
Templates, Genetic

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.