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Article

A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

Gene - 30 Nov 1990

Landt O, Grunert H P, Hahn U

Abstract excerpt

We have developed a general and rapid method for site-directed mutagenesis using primed amplification by the polymerase chain reaction. Starting from a double-stranded DNA template, this method requires only one single specific mutagenic primer and two universal sequencing primers flanking the region to be mutated further upstream and downstream, respectively. To test the method, two different mutants of the...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 2265750
DOI: 10.1016/0378-1119(90)90351-q

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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