Article
Hypermutagenic in vitro transcription employing biased NTP pools and manganese cations.
Gene - 20 Feb 1997
Pezo V, Wain-Hobson S
Abstract excerpt
In vitro DNA-dependent RNA transcription using bacteriophage T3 RNA polymerase may be rendered hypermutagenic by employing biased nucleoside triphosphate (NTP) concentrations and manganese cations. Using the E. coli R67 plasmid-encoded dihydrofolate reductase (DHFR) gene as target substitution rates approaching 4 x 10(-2) per base per reaction could be achieved, on a par with hypermutagenic reverse transcription....
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