Article
Interactions of Arg2 in the Mnt N-terminal arm with the central and flanking regions of the Mnt operator.
Journal of molecular biology - 25 Aug 2000
Berggrun A, Sauer R T
Abstract excerpt
Arg2, in the N-terminal arm of the Mnt repressor, plays an important role in determining operator-binding specificity. In the complex of the Mnt tetramer with the 21 base-pair mnt operator, there are four potential sites for Arg2 interactions, two in the central region of the operator and two on the outer flanks of the operator. Single-chain variants of the dimeric N-terminal domain of Mnt containing one Arg2...
Topics
- Base Sequence
- DNA Footprinting
- DNA, Viral
- DNA-Binding Proteins
- Dimerization
- Edetic Acid
- Ferric Compounds
- Genes, Viral
- Models, Molecular
- Mutation
- Oligodeoxyribonucleotides
- Operator Regions, Genetic
- Protein Binding
- Protein Structure, Tertiary
- Recombinant Proteins
- Repressor Proteins
- Response Elements
- Substrate Specificity