Article
Multifocal two-photon laser scanning microscopy combined with photo-activatable GFP for in vivo monitoring of intracellular protein dynamics in real time.
Journal of structural biology - 1 Jun 2007
Martini Joerg, Schmied Katja, Palmisano Ralf, Toensing Katja, Anselmetti Dario, Merkle Thomas
Abstract excerpt
We used multifocal two-photon laser scanning microscopy for local and selective protein activation and quantitative investigation of intracellular protein dynamics. The localized activation was realized with photo-activatable green-fluorescent-proteins (pa-GFP) and optical two-photon excitation in order to investigate the real-time intracellular dynamics in vivo. Such processes are of crucial importance for a...
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