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Article

Re-assessment of biolistic transient expression: An efficient and robust method for protein localization studies in seedling-lethal mutant and juvenile plants.

Plant science : an international journal of experimental plant biology - 1 Sept 2018

Wang Hao, Zhao Qiong, Fu Jiaxin, Wang Xiangfeng, Jiang Liwen

Abstract excerpt

Knowledge on the subcellular localization of target proteins in a plant mutant background is important for revealing the function of the genes investigated. However, in Arabidopsis and rice, mutant lethality is one major barrier to such studies. Here we describe an optimized bombardment-mediated transient expression approach for studying subcellular protein localization in Arabidopsis seedling of lethal mutants....

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PMID: 30080604
DOI: 10.1016/j.plantsci.2018.03.032

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