Article
Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells.
Nature communications - 14 Jul 2014
Niopek Dominik, Benzinger Dirk, Roensch Julia, Draebing Thomas, Wehler Pierre, Eils Roland, Di Ventura Barbara
Abstract excerpt
The function of many eukaryotic proteins is regulated by highly dynamic changes in their nucleocytoplasmic distribution. The ability to precisely and reversibly control nuclear translocation would, therefore, allow dissecting and engineering cellular networks. Here we develop a genetically encoded, light-inducible nuclear localization signal (LINuS) based on the LOV2 domain of Avena sativa phototropin 1. LINuS is...
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