Article
Visualizing single molecules inside living cells using total internal reflection fluorescence microscopy.
Methods (San Diego, Calif.) - 1 Feb 2003
Mashanov G I, Tacon D, Knight A E, Peckham M, Molloy Justin E
Abstract excerpt
Over the past 10 years, advances in laser and detector technologies have enabled single fluorophores to be visualized in aqueous solution. Here, we describe methods based on total internal reflection fluorescence microscopy (TIRFM) that we have developed to study the behavior of individual protein molecules within living mammalian cells. We have used cultured myoblasts that were transiently transfected with DNA...
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