Article
Genotypic analysis of mutations in Taq I restriction recognition sites by restriction fragment length polymorphism/polymerase chain reaction.
Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1992
Sandy M S, Chiocca S M, Cerutti P A
Abstract excerpt
Point mutations in somatic cells play a role in the etiology of several classes of human pathologies. Experimental procedures are required that allow the detection and quantitation of such mutations in disease-related genes in tissue biopsy samples without the need for the selection of mutated cells. We describe the genotypic analysis of single base pair mutations in the Taq I endonuclease recognition sequence...
Topics
- Base Sequence
- Deoxyribonucleases, Type II Site-Specific
- Genes, ras
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Polymorphism, Genetic