Article
Genotypic analysis of N-ethyl-N-nitrosourea-induced mutations by Taq I restriction fragment length polymorphism/polymerase chain reaction in the c-H-ras1 gene.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jun 1992
Chiocca S M, Sandy M S, Cerutti P A
Abstract excerpt
In genotypic mutation analysis DNA sequence changes are determined without the in vivo or in vitro selection of phenotypically altered cells. We have studied the induction of base-pair changes by N-ethyl-N-nitrosourea in Taq I endonuclease recognition site 2508-2511 (TCGA) of the c-H-ras1 gene in...
Topics
- Base Sequence
- Cells, Cultured
- DNA
- DNA-Directed DNA Polymerase
- Ethylnitrosourea
- Fibroblasts
- Genes, ras
- Genotype
- Humans
- Molecular Sequence Data
- Mutagenesis
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Skin
- Taq Polymerase