Article
Inclusion of synthetic DNA templates of similar length and base composition to PCR-amplified products in restriction enzyme digestions: an efficient aid in characterization of point mutations.
Somatic cell and molecular genetics - 1 Jan 1994
Klein D, Tabor D E, Sokol R Z, Cederbaum S D
Abstract excerpt
Because of a subtle anomaly we encountered upon an analytical gel while characterizing a point mutation in an exon of a patient, we decided to perform expensive and time-consuming procedures to characterize the anomaly. Although initial and subsequent Southern blots and PCR analyses of this patie...
Topics
- Alleles
- Base Composition
- DNA
- DNA Mutational Analysis
- DNA Restriction Enzymes
- Humans
- Point Mutation
- Polymerase Chain Reaction
- Templates, Genetic