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Article

A genotypic mutation system measuring mutations in restriction recognition sequences.

Nucleic acids research - 11 Jun 1991

Felley-Bosco E, Pourzand C, Zijlstra J, Amstad P, Cerutti P

Abstract excerpt

The RFLP/PCR approach (restriction fragment length polymorphism/polymerase chain reaction) to genotypic mutation analysis described here measures mutations in restriction recognition sequences. Wild-type DNA is restricted before the resistant, mutated sequences are amplified by PCR and cloned. We tested the capacity of this experimental design to isolate a few copies of a mutated sequence of the human c-Ha-ras1...

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Identifiers

PMID: 1676153
DOI: 10.1093/nar/19.11.2913
PMCID: PMC328251

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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