Article
A genotypic mutation system measuring mutations in restriction recognition sequences.
Nucleic acids research - 11 Jun 1991
Felley-Bosco E, Pourzand C, Zijlstra J, Amstad P, Cerutti P
Abstract excerpt
The RFLP/PCR approach (restriction fragment length polymorphism/polymerase chain reaction) to genotypic mutation analysis described here measures mutations in restriction recognition sequences. Wild-type DNA is restricted before the resistant, mutated sequences are amplified by PCR and cloned. We tested the capacity of this experimental design to isolate a few copies of a mutated sequence of the human c-Ha-ras1...
Topics
- Base Sequence
- DNA-Directed DNA Polymerase
- Genotype
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Taq Polymerase