Article
Subcellular trafficking of the yeast plasma membrane ABC transporter, Pdr5, is impaired by a mutation in the N-terminal nucleotide-binding fold.
Molecular microbiology - 1 Feb 2007
de Thozée Cédric Pety, Cronin Susan, Goj Agnieszka, Golin John, Ghislain Michel
Abstract excerpt
The plasma membrane ATP-binding cassette (ABC) transporter, Pdr5p, mediates resistance to many different xenobiotic compounds in yeast. We have isolated several mutated forms that fail to confer resistance to cycloheximide and itraconazole. Here, we examined two variants, the expression of which was abnormally low when cells reach the stationary phase of growth. The Pdr5(1157) variant lacked the C-terminal...
Topics
- ATP-Binding Cassette Transporters
- Amino Acid Substitution
- Cell Membrane
- Codon, Nonsense
- Cycloheximide
- Drug Resistance, Fungal
- Endoplasmic Reticulum
- Microscopy, Fluorescence
- Mutation
- Protein Folding