Article
Activities of native and tyrosine-69 mutant phospholipases A2 on phospholipid analogues. A reevaluation of the minimal substrate requirements.
Biochemistry - 26 Jun 1990
Kuipers O P, Dekker N, Verheij H M, de Haas G H
Abstract excerpt
The role of Tyr-69 of porcine pancreatic phospholipase A2 in substrate binding was studied with the help of proteins modified by site-directed mutagenesis and phospholipid analogues with a changed head-group geometry. Two mutants were used containing Phe and Lys, respectively, at position 69. Mod...
Topics
- Animals
- Base Sequence
- Esters
- Lysine
- Molecular Sequence Data
- Mutation
- Pancreas
- Phenylalanine
- Phosphates
- Phosphatidylcholines
- Phospholipases
- Phospholipases A
- Phospholipases A2
- Phospholipids
- Protein Binding
- Structure-Activity Relationship
- Substrate Specificity
- Swine