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Article

Production in Escherichia coli, folding, purification and characterization of notexin with wild type sequence and with N-terminal and catalytic site mutations.

Toxicon : official journal of the International Society on Toxinology - 1 Sept 2014

Simonato Morena, Morbiato Laura, Zorzi Veronica, Caccin Paola, Fernández Julián, Massimino Maria L, Polverino de Laureto Patrizia, Tonello Fiorella

Abstract excerpt

Notexin (Ntx) is a group I phospholipase A2 (PLA2) protein, main component of the Australian snake Notechis scutatus scutatus venom. It is both a presynaptic neurotoxin and a myotoxin. In this work, for the first time, a method for the production and folding of recombinant Ntx was developed. Ntx was produced with wild type sequence (rNtx), with an extra peptide (T7-Ntx) or a methionine (M-Ntx) before Asn-1, and...

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Identifiers

PMID: 24951874
DOI: 10.1016/j.toxicon.2014.06.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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