Article
Mutations on N-terminal region of Taiwan cobra phospholipase A(2) result in structurally distorted effects.
Journal of peptide science : an official publication of the European Peptide Society - 1 Aug 2008
Chiou Yi-Ling, Lin Shinne-Ren, Chang Long-Sen
Abstract excerpt
In the present study, three Taiwan cobra PLA(2) variants were prepared by adding an extra N-terminal Met, substituting Asn-1 by Met or deleting the N-terminal heptapeptide. Recombinant PLA(2) mutants were expressed in Escherichia coli (E. coli), and purified to homogeneity by reverse phase HPLC....
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