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Article

Mutations on N-terminal region of Taiwan cobra phospholipase A(2) result in structurally distorted effects.

Journal of peptide science : an official publication of the European Peptide Society - 1 Aug 2008

Chiou Yi-Ling, Lin Shinne-Ren, Chang Long-Sen

Abstract excerpt

In the present study, three Taiwan cobra PLA(2) variants were prepared by adding an extra N-terminal Met, substituting Asn-1 by Met or deleting the N-terminal heptapeptide. Recombinant PLA(2) mutants were expressed in Escherichia coli (E. coli), and purified to homogeneity by reverse phase HPLC. Fluorescence measurement showed that the hydrophobic character of the catalytic site, the microenvironment of Trp...

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PMID: 18288787
DOI: 10.1002/psc.1020

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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