Article
Identification of a translocated gating charge in a voltage-dependent channel. Colicin E1 channels in planar phospholipid bilayer membranes.
The Journal of general physiology - 1 Jul 1991
Abrams C K, Jakes K S, Finkelstein A, Slatin S L
Abstract excerpt
The availability of primary sequences for ion-conducting channels permits the development of testable models for mechanisms of voltage gating. Previous work on planar phospholipid bilayers and lipid vesicles indicates that voltage gating of colicin E1 channels involves translocation of peptide segments of the molecule into and across the membrane. Here we identify histidine residue 440 as a gating charge...
Topics
- Base Sequence
- Colicins
- Hydrogen-Ion Concentration
- Ion Channel Gating
- Ion Channels
- Kinetics
- Membranes, Artificial
- Molecular Sequence Data
- Mutation
- Phospholipids
- Potassium Chloride