Article
Protein translocation across planar bilayers by the colicin Ia channel-forming domain: where will it end?
The Journal of general physiology - 1 Oct 2000
Kienker P K, Jakes K S, Finkelstein A
Abstract excerpt
Colicin Ia, a 626-residue bactericidal protein, consists of three domains, with the carboxy-terminal domain (C domain) responsible for channel formation. Whole colicin Ia or C domain added to a planar lipid bilayer membrane forms voltage-gated channels. We have shown previously that the channel formed by whole colicin Ia has four membrane-spanning segments and an approximately 68-residue segment translocated...
Topics
- Animals
- Colicins
- Escherichia coli
- Indicators and Reagents
- Ion Channel Gating
- Ion Channels
- Lipid Bilayers
- Mutation
- Protein Transport
- Streptavidin