Identification and biochemical analysis of a mitochondrial endonuclease of Podospora anserina related to curved-DNA binding proteins.

doi:10.1016/j.bbagen.2006.10.003

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Article

Identification and biochemical analysis of a mitochondrial endonuclease of Podospora anserina related to curved-DNA binding proteins.

Biochimica et biophysica acta - 1 Apr 2007

Laquel-Robert Patricia, Sellem Carole H, Sainsard-Chanet Annie, Castroviejo Michel

Abstract excerpt

We purified and characterized previously from Podospora anserina mitochondria an endonuclease, active on single-stranded, double-stranded and flap DNA, with RNAse H activity, named P49 according to the major 49 kDa band observed on SDS-PAGE. Edman sequencing allowed us to identify the corresponding gene called nuc49. Here we report the properties of the (His)-tagged NUC49 protein expressed in E. coli. We show...

Topics

Amino Acid Sequence
Bacteriophage M13
Base Sequence
Cations
DNA, Circular
DNA, Single-Stranded
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
Flap Endonucleases
Fungal Proteins
Mitochondria
Molecular Sequence Data
Molecular Weight

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Podospora

Recombinant Proteins

Sequence Alignment

Sequence Analysis, DNA

Source

Open in PubMed

Identifiers

PMID: 17188431
DOI: 10.1016/j.bbagen.2006.10.003

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