Article
A positively charged residue of phi29 DNA polymerase, highly conserved in DNA polymerases from families A and B, is involved in binding the incoming nucleotide.
Nucleic acids research - 1 Apr 2002
Truniger Verónica, Lázaro José M, Esteban Francisco J, Blanco Luis, Salas Margarita
Abstract excerpt
Alignment of the protein sequence of DNA-dependent DNA polymerases has allowed the definition of a new motif, lying adjacent to motif B in the direction of the N-terminus and therefore named pre-motif B. Both motifs are located in the fingers subdomain, shown to rotate towards the active site to form a dNTP-binding pocket in several DNA polymerases in which a closed ternary complex pol:DNA:dNTP has been solved....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.