Article
Disruption of the LF-A1 and LF-B1 binding sites in the human alpha-1-antitrypsin gene has a differential effect during development in transgenic mice.
The EMBO journal - 1 Nov 1991
Tripodi M, Abbott C, Vivian N, Cortese R, Lovell-Badge R
Abstract excerpt
Previous work in transfected cell lines and in nuclear extracts has led to the identification of two cis-acting elements important for transcription of the human alpha-1-antitrypsin (A1AT) gene, which bind to two liver specific trans-acting factors, LF-A1 and LF-B1. Mutations EM3 and PM1, which abolish the binding of LF-A1 and LF-B1 respectively, drastically reduce transcription activity of the A1AT gene in vitro...
Topics
- Aging
- Animals
- Binding Sites
- Blotting, Northern
- Chloramphenicol O-Acetyltransferase
- Cosmids
- DNA
- DNA-Binding Proteins
- Hepatocyte Nuclear Factor 1
- Hepatocyte Nuclear Factor 1-alpha