Article
Regulation of the mouse alpha A-crystallin gene: isolation of a cDNA encoding a protein that binds to a cis sequence motif shared with the major histocompatibility complex class I gene and other genes.
Molecular and cellular biology - 1 Jul 1990
Nakamura T, Donovan D M, Hamada K, Sax C M, Norman B, Flanagan J R, Ozato K, Westphal H, Piatigorsky J
Abstract excerpt
We have shown by site-directed mutagenesis that the sequence between positions -69 and -40 of the mouse alpha A-crystallin gene is crucial for tissue-specific gene expression in a transfected mouse lens epithelial cell line transformed with the early region of simian virus 40. Gel retardation experiments with synthetic oligodeoxynucleotides revealed a mouse lens nuclear protein which bound specifically to the...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cell Line
- Crystallins
- DNA
- DNA-Binding Proteins
- Epithelium
- Gene Expression Regulation