Article
Sequence-specific modification of mitochondrial DNA using a chimeric zinc finger methylase.
Proceedings of the National Academy of Sciences of the United States of America - 26 Dec 2006
Minczuk Michal, Papworth Monika A, Kolasinska Paulina, Murphy Michael P, Klug Aaron
Abstract excerpt
We used engineered zinc finger peptides (ZFPs) to bind selectively to predetermined sequences in human mtDNA. Surprisingly, we found that engineered ZFPs cannot be reliably routed to mitochondria by using only conventional mitochondrial targeting sequences. We here show that addition of a nuclear export signal allows zinc finger chimeric enzymes to be imported into human mitochondria. The selective binding of...
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