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Article

Biotin-psolaren labeled cDNA amplicons for genotyping rotavirus strains by dot hybridization assay.

Journal of virological methods - 1 Mar 2007

Domingues André Luiz S, Silva Maria Helena, Gouvea Vera

Abstract excerpt

A simple method to produce biotinylated probes by utilizing an amine-psoralen to conjugate an ester-biotin to nucleic acid molecules is described. It is simple, rapid, and well suited to label cDNA probes with any PCR-generated amplicon for hybridization assay. Its application to identify or to confirm the G and P genotypes of rotavirus-derived amplicons is described; however, it may be used to label amplicons of...

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Source

Identifiers

PMID: 17169439
DOI: 10.1016/j.jviromet.2006.11.016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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