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Cloning of noncultivatable human rotavirus by single primer amplification

1 Mar 1992

Abstract excerpt

A novel, sequence-independent strategy has been developed for the amplification of full-length cDNA copies of the genes of double-stranded RNA (dsRNA) viruses. Using human (Bristol) group C rotavirus as an example, a single amino-linked modified oligonucleotide (primer 1) was ligated to either end of each dsRNA genome segment by using T4 RNA ligase. Following reverse transcription, annealing, and repair of cDNA...

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PMID: 1371174
DOI: 10.1128/jvi.66.3.1817-1822.1992

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