Detection and genotyping of human group A rotaviruses by oligonucleotide microarray hybridization.

doi:10.1128/JCM.40.7.2398-2407.2002

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Article

Detection and genotyping of human group A rotaviruses by oligonucleotide microarray hybridization.

Journal of clinical microbiology - 1 Jul 2002

Chizhikov V, Wagner M, Ivshina A, Hoshino Y, Kapikian A Z, Chumakov K

Abstract excerpt

A rapid and reliable method for the identification of five clinically relevant G genotypes (G1 to G4 and G9) of human rotaviruses based on oligonucleotide microarray hybridization has been developed. The genotype-specific oligonucleotides immobilized on the surface of glass slides were selected to bind to the multiple target regions within the VP7 gene that are highly conserved among individual rotavirus...

Topics

Antigens, Viral
Base Sequence
Capsid
Capsid Proteins
DNA Primers
DNA, Viral
Drug Design
Genes, Viral
Genotype
Humans
Molecular Probe Techniques
Mutation
Oligonucleotide Array Sequence Analysis
Oligonucleotide Probes
Rotavirus

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Identifiers

PMID: 12089254
DOI: 10.1128/JCM.40.7.2398-2407.2002
PMCID: PMC120567

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Species Specificity

Virology