Article
Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization.
Journal of clinical microbiology - 1 May 1990
Chapman N M, Tracy S, Gauntt C J, Fortmueller U
Abstract excerpt
Analysis of enteroviral genomes has revealed that the 5' nontranslated region is highly conserved, providing consensus sequences for the design of oligonucleotides which should anneal to most, if not all, human enteroviral RNAs. We designed and used a pair of such generic primers to enzymatically...
Topics
- Base Sequence
- DNA
- DNA Probes
- Enterovirus
- Enterovirus Infections
- Gene Amplification
- Genotype
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
